Research & Test Info

The independent lab tests and studies below were done exclusively on Norwex Microfiber products throughout the world.  The results are very impressive and nothing short of amazing.  I encourage you to read through these thoroughly.  Any further questions regarding these tests can be emailed directly to Norwex at   info@norwex.com   I also would like to encourage you to research the internet for yourself regarding both the home and professional product lines that Norwex offers.  Their Professional Product Line is currently be used in Hospitals, Medical facilities, Schools, Hotels, Daycares, Adult Care Centers, Restaurants, Hair and Nail Salons, Offices, Etc. throughout Europe and Asia.

 

TO VIEW THE SWEDISH STUDY CLICK THE LINK BELOW

www.norwex-healthy-cleaning.citymax.com/f/20050523_Bacteria_tests_Superior_cloth.pdf

VIEW THE LUND UNIVERSITY STUDY CLICK THE LINK BELOW

www.norwex-healthy-cleaning.citymax.com/f/New_hospital_testing.doc

TO VIEW THE BIRD FLU TEST RESULTS CLICK THE LINK BELOW

www.norwex-healthy-cleaning.citymax.com/f/bird_flu_final_report_edited.doc

 

 THE SARS TEST RESULTS:

 

 

Test Summary

                 of Inactivating Extrasomatic SARS Virus

With Norwex Inorganic Antibacterial Agent

 

 

 

 

 

 

 

 

 

Person in Charge of the Test Design: Duan Shumin

 

Test participators: Duan Shumin, Zhao Xinsheng, Wen Ruifu & Huang Jingjing

 

Test Date: July 2003 ~ Oct. 2003

 

Test Organization: The Virosis Prevention Control Institute of ChineseCenter for Disease Control and Prevention

 

Liaison tel. for Test Data: 010-63536459

 

Commissioning Organization: Beijing Great Wall Yongyi Science & Technology Development Co., Ltd.

 

 

 

 

 

 

 

 

 

The Virosis Prevention Control Institute of Chinese Center for Disease Control and Prevention

 

July 2003

Test Summary of Inactivating Extrasomatic SARS Virus

With NORWEX Inorganic Antibacterial Agent

 

Abstract:

Checking effect to inactivate extrasomic SARS virus in the substrate of VERO E6with NORWEX inorganic antibacterial agent by adopting virus (CPE) method, the test result manifests that the NORWEX Inorganic antibacterial agent has a certain inactivation effect upon SARS virus after it functions in the room temperature for over 6h.

 

  1. Test Purpose

 

The test purpose is to detect whether the NORWEX inorganic antibacterial agent possesses inactivation effect upon SARS virus under the condition of ambient temperature and functions on SARS virus for different time duration, and provide test basis for accelerating the progress rate in sifting the stuff for inactivating SARS virus.

 

  1. Test Materials

 

1)      Verification Material:

NORWEX inorganic antibacterial agent: White powder, 10g per bag, provided by Beijing Great Wall Yongyi Science & Technology Development Co., Ltd.

 

2)      Positive Comparison Medicament:

GANCICLOVIR for Injection; Batch No. 020802; Provided by Hubei Keyi Pharmaceutic Co., Ltd.

 

3)      Virus:

SARS-COV-P11 corona-virus separated strain (No.11 specimen of SARS patient blood serum) provided by You’an Hospital; Separated and identified by this room.

  

SARS-COV-P8 corona-virus separated strain, provided by (Professor Li Dexin) the Hemorrhagic Fever Room of Virosis Prevention Control Institute.

 

4)      Cells:

The generation spreading kidney cells of African green monkey(VERO E6), provided by this room.

 

5)      The test materials, such as cells culture maintenance media “Eagle’s” etc, were provided by this room.

 

 

  1. Experimental Method

 

Preliminary Test

 

1)      Toxicity determination for VERO E6 cells by using NORWEX inorganic antibacterial agent:

Calculate the half toxic concentration (TD50) and maximum non-poisonous concentration (TD0) through VERO E6 cell culture in substrate, by adopting cellular morphological variation (CPE) method and Reed-Muenchdrug method.

 

The pretreatment of the NORWEX inorganic antibacterial agent:

Put 6000μg/ml NORWEX inorganic antibacterial agent into a vessel and have the antibacterial agent diluted in Eagle’s cells maintenance media; then lay the vessel on a magnetic stirrer (in ambient temperature); sample from the vessel at separate agitating time, i.e. at 2h, 4h and 6h from the starting time of the agitation. Each specimen sampled at different time should be diluted, i.e. 6000μ g/ml ~ 187.5μ g/ml, and inoculate VERO E6 cells into culture board of 96 orifices.

 

Cellular morphological variation (CPE) method:

Inoculate the VERO E6 cells with the concentration of 400 thousand unit/ml into the culture board of 96 orifices, and have the object cultured under the condition of 37℃ and 5% CO2 for 24h till  cellular mono-layer; Then add the pretreated NORWEX inorganic antibacterial agent, the concentration of which is 6,000μg/ml ~187.5μ g/ml; the multiple proportion of the positive comparison medicament is 6,000μ g/ml ~ 187.5μ g/ml, and that in each kind of the concentrations is to be inoculated into four orifices with 100μl/orifice. Meanwhile, normal cellular is to be assumed for control. Lay the normal cells in the environment of 37℃ and 5% CO2 to culture for 5~7 days, and observe and take down the cellular morphological variation (CPE) every 24h under inverted microscope: Denominate the variation under 25% as “+”, the variation of 26% ~ 50% as ” ++”, the variation of 51% ~ 75% as “+++”, and the variation of 76% ~ 100% as ” ++++”. The test should be repeated for tree times.

 

2)      Separate and identify the SARS-COV-P11 in VERO E6 cells culture substrate

 

SARS-COV-P11 (Separation of No 11 SARS patient blood serum):

Inoculate the VERO E6 cells in the concentration of 400 thousand unit/ml into test tube, and have the cells cultured in the environment of 37℃ and 5% CO2 for 24h; Then discard the culture solution and add the 0.2ml blood serum of SARS patient into each test tube; after the objects are cultured in rotary drum for 5h, add 1ml maintenance media. At the same time, the normal cells control should be assumed; the ambient temperature is 37℃ and the culturing duration in the rotary drum is 5 ~ 7 days. After the CPE variation occurs in the cells, detect corona-virus by PCR method. If the specimen No. 11 PCR detection presents “Positive”, it can be determined as the corona-virus separate strain. The virus should be purified twice with final dilution method, and then conduct PCR detection. If the S gene sequence of the SARS virus at this time still presents positive, and at the same time if IgM is positive and IgG4 value goes up by times through immuno-fluorescent assay of double SARS patient blood serums, it can be determined as corona-virus. CPE method should be adopted to determine its potency.

 

Determine the toxicity of SARS-COV-P1be & SARS-COV-P8 toxicant strain in VERO E6 cells culture substrate

 

Virus CPE Method:

Inoculate the VERO E6 cells in the concentration of 400 thousand unit/ml into culture board of 96 orifices, and have the cells cultured in the environment of 37℃and 5% CO2 for 24h; Then discard the culture solution and dilute 2 strains of the virus respectively into eight kinds of concentrations from 10-1 to 10-8. Each kind of the concentration should occupy 4 orifices and each orifice holds 100μL solution. Meanwhile, the control of normal cells should be cultured for 5 ~ 7 days in the environment of 37℃ and 5% CO2. Observe and take down the cellular morphological variation (CPE) under inverted microscope once every 24h: Denominate the variation under 25% as “+”, the variation of 26% ~ 50% as ” ++”, the variation of 51% ~ 75% as “+++”, and the variation of 76% ~ 100% as ” ++++”. Calculate the median toxic concentration TCID50 by Reed –Muench Method.

 

3)      The inactivating effect of NORWEX inorganic antibacterial agent in VERO E6 cells culture substrate upon SARS-COV-P11 and SARS-COV-P8

 

Test Purposes:

Virus cells (CPE) method should be adopted to observe the inactivating effect of the NORWEX inorganic antibacterial agents in different concentrations upon the SARS viral in the VERO E6 cell culture substrate, and calculate the median effective concentration (IC50) and the minimum effective concentration (MIC), as well as the therapeutic index (TI) and determine the potency.

 

Pretreatment of 100TCID50 SARS virus & NORWEX inorganic antibacterial agent:

Put two strains of 100TCID50SARS virus diluents and 750μg/ml NORWEX inorganic antibacterial agent into a vessel respectively. After having them agitated in magnetic stirrer for 2h, 4h and 6h, sample object respectively and dilute the specimens into 750μ g/ml ~ 11.7μ g/ml, then inoculate VERO E6 cells into the culture board of 96 orifices.

 

Virus CPE Method:

Inoculate the VERO E6 cells in the concentration of 400 thousand unit/ml into culture board of 96 orifices, and have the cells cultured in the environment of 37℃and 5% CO2 for 24h till mono-layer; Then discard the culture solution and add the pretreated solution with SARS virus and NORWEX inorganic antibacterial agent; select two times of the maximum non-poisonous concentration (TD0) to the cells to be diluted for 7 grade of thickness, i.e. 750μg/ml ~μ g/ml; meanwhile, the pretreated control of NORWEX inorganic antibacterial agent should be two times diluted into 7 grades of thickness, i.e. 750μ g/ml ~ 5.9μ g/ml; take the GANCICLOVIR for Injection as the positive comparison medicament, which would have the maximum non-poisonous concentration (TD0) to the cells two times diluted into 7 grades of concentrations, i.e.6000μ g/ml ~ 5.9 μ g/ml. Add the diluted medicament into the cell orifices respectively; each grade of the concentration should occupy 4 orifices. At the same time, the normal cells control should be assumed; the ambient temperature is 37℃ and the culturing duration in the rotary drum is 5 ~ 7 days; Observe the virus CPE under inverted microscope every day by comparison. When “+++” ~ “++++” occurs, end off the test. Calculate the median effective concentration(IC50), the minimum effective concentration (MIC) and the therapeutic index (TI) of the medicament to decide the effect by Reed – Muench method. The test should be repeated for tree times.

  

  1. Test Result

  

Preliminary Test Result:

  

The toxic action of NORWEX inorganic antibacterial agent upon VERO E6:

 

Calculate the maximum nontoxic concentration (TD0) and median toxic concentration (TD50) of the NORWEX inorganic antibacterial agent and positive comparison medicament i.e. the GANCICLOVIR for injection in VERO E6 cells culture substrate by adopting Cellular morphological variation (CPE) method provided by Beijing Great Wall Yongyi Science & Technology Development Co., Ltd. And the following test data is the average values of thrice test results.

 

1)      The test result for the toxicity of NORWEX inorganic antibacterial agent to the VERO E6 cells

 

Verification Material:

 

NORWEX inorganic antibacterial agent: The maximum nontoxic concentration(TD0)is 750±0 μ g/ml and the medium toxic concentration(TD50)is 1500±0 μ g/ml.

 

Positive comparison medicament:

 

GANCICLOVIR for injection: The maximum nontoxic concentration(TD0)is >6000±0 μ g/ml, and the medium toxic concentration(TD50)is >6000±0 μg/ml.

 

2)      The test result for the toxicity in VERO E6 cells culture substrate to SARS-COV-P11 & SARS-COV-P8

 

SARS-COV-P11: Median infective dose(TCID50)is 10– 7

SARS-COV-P8: Median infective dose(TCID50)is 10– 7

 

Official Test Result:

 

1)   After the NORWEX inorganic antibacterial agent takes action at the SARS-COV-P11 for 2h, 4h and 6h, the inactivation effect could be checked in the VERO E6 cells culture substrate, by taking IC50, MIC, TI and the percentage of inactivation as the indices (The following test data are the average values of thrice test results).

 

Detect the inactivation effect (taking IC50, MIC and TI as the indices).

 

Verification Material:

 

NORWEX inorganic antibacterial agent:

After the medicament action for 2h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 188±0 μg/ml, and the minimum effective concentration(MIC)is 94±0 μ g/ml; the therapeutic index(TI)is 8.

 

After the medicament action for 4h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 188±0 μ g/ml, and the minimum effective concentration(MIC)is 94±0 μ g/ml; the therapeutic index(TI)is 8.

 

After the medicament action for 6h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 94±0 μ g/ml, and the minimum effective concentration(MIC)is 46.8±0 μ g/ml; the therapeutic index(TI)is 16.

 

Detection of inactivation effect (Taking inactivation percentages as the indices):

After the medicament action for 2h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >375μg/ml, the 100% SARS virus can be inactivated; the diluted concentration is 188μ g/ml, 50% SARS virus can be inactivated; when the diluted concentration is 94μ g/ml, 25% SARS virus can be inactivated.

 

After the medicament action for 4h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >375μg/ml, the 100% SARS virus can be inactivated; the diluted concentration is 188μ g/ml, 50% SARS virus can be inactivated; when the diluted concentration is 94μ g/ml, 25% SARS virus can be inactivated.

 

After the medicament action for 6h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >188μg/ml, the 100% SARS virus can be inactivated; when the diluted concentration is 94μg/ml, 50% SARS virus can be inactivated; When the diluted concentration is 46.8μ g/ml, 25% SARS virus can be inactivated.

 

Positive comparison medicament:

GANCICLOVIR for injection: With virus CPE method, median effective concentration(IC50)is 11.7±0 μ g/ml, and the minimum effective concentration(MIC)is 23.44±0 μ g/ml; the therapeutic index(TI)is 256.

 

2)   After the NORWEX inorganic antibacterial agent takes action at the SARS-COV-P8 for 2h, 4h and 6h, the inactivation effect could be checked in the VERO E6 cells culture substrate, by taking IC50, MIC, TI and the percentage of inactivation as the indices (The following test data are the average values of thrice test results).

 

Detect the inactivation effect (taking IC50, MIC and TI as the indices).

 

Verification Material:

 

NORWEX inorganic antibacterial agent:

After the medicament action for 2h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 188±0 μ g/ml, and the minimum effective concentration(MIC)is 94±0 μ g/ml; the therapeutic index(TI)is 8.

 

After the medicament action for 4h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 188±0 μ g/ml,and the minimum effective concentration(MIC)is 94±0 μ g/ml; the therapeutic index(TI)is 8.

 

After the medicament action for 6h under ambient temperature:

With virus CPE method, median effective concentration(IC50)is 94±0 μ g/ml, and the minimum effective concentration(MIC)is 46.8±0 μ g/ml; the therapeutic index(TI)is 16.

 

Detection of inactivation effect (Taking inactivation percentages as the indices):

After the medicament action for 2h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >375μg/ml, the 100% SARS virus can be inactivated; the diluted concentration is 188μ g/ml, 50% SARS virus can be inactivated; when the diluted concentration is 94μ g/ml, 25% SARS virus can be inactivated.

 

After the medicament action for 4h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >375μg/ml, the 100% SARS virus can be inactivated; the diluted concentration is 188μ g/ml, 50% SARS virus can be inactivated; when the diluted concentration is 94μ g/ml, 25% SARS virus can be inactivated.

 

After the medicament action for 6h under ambient temperature:

With virus CPE method, when the diluted concentration of the NORWEX inorganic antibacterial agent >188μg/ml, the 100% SARS virus can be inactivated; when the diluted concentration is 94μg/ml, 50% SARS virus can be inactivated; When the diluted concentration is 46.8μ g/ml, 25% SARS virus can be inactivated.

 

Positive comparison medicament:

GANCICLOVIR for injection: With virus CPE method, median effective concentration(IC50)is 11.7±0 μ g/ml, and the minimum effective concentration(MIC)is 23.44±0 μ g/ml; the therapeutic index(TI)is 256.

 

 

Summary

 

With the inorganic antibacterial agent and the positive comparison medicament i.e. GANCICLOVIR for injection provided by Beijing Great Wall Yongyi Science & Technology Co. Ltd. in VERO E6 cell culture substrate, while adopting virus CPE method, the result, verified by the tests with two SARS virus isolated strains, indicates that the NORWEX inorganic antibacterial agent has definite inactivation effect upon the SARS virus if it acts with the SARS virus for over 6h in ambient temperature.

 

 

 

Person in Charge of the Test Design: Duan Shumin

 

Test participators: Duan Shumin, Zhao Xinsheng, Wen Ruifu & Huang Jingjing

 

Test Date: July 2003 ~ Oct. 2003

 

Test Organization: The Virosis Prevention Control Institute of ChineseCenter for Disease Control and Prevention

 

Liaison tel. for Test Data: 010-63536459

 

Commissioning Organization: Beijing Great Wall Yongyi Science & Technology Development Co., Ltd

 THE MRSA & E. COLI TEST RESULTS – JAPAN :

 

 

 

 

 

 

Norwex Japan Co. Ltd.                                                                    Date of Issue:  May 16, 2003

 

 

Japan Spinners Inspecting Foundation

Kinki Head Office

1-18-15 Ue-machi, Chuo-ku, Osaka 540-0005, Japan

Tel:  06-6762-5887

Fax:  06-6762-8588

 

Date of sample receipt:  April 30, 2003                          Test No. 604738

Article:  cloth

Subject:   Test for microorganism eradication

Tested Culture:   Staphylococcus aureus      NBRC 12732

Escherichia coli                  NBRC 3972

 

Test Method:

Culture solution regulated at 2.5 to 10 x 10 cells/ml is dropped on a film (6 x 6 cm square) by 0.4ml using 1/500NB. The culture solution on the film is wiped off by the cloth sample. Then detection on the film surface is performed immediately to measure viable cell count by mixed dilution plate medium culture method. Besides, measurement on another film is also conducted after dropping culture solution in order to exemplify viable cell count before wiping off. The rate of eradication is calculated according to the formula below.

 

Rate of eradication (%)

Name of sample Staphylococcus aureus Escherichia coli
       Cloth 99.9% or more  99.9% or more

 

 

Date of sample receipt:  September 3, 2003

Subject: Test for microorganism eradication

Tested Culture:   Staphylococcus aureus      NBRC 12732

Escherichia coli                  NBRC 3972

 

Test Method:

The above test was completed again in the same manner. However this test was conducted after the cloth sample had been washed 300 times based on the washing resistance

 

Rate of eradication (%)

Name of sample Staphylococcus aureus Escherichia coli
       Cloth 99.9% or more  99.9% or more

 

This test confirms that the Norwex Microfiber cloth removes 99.9% of bacteria from a surface.

It also confirmed that even after washing the cloth 300 times it was still able to pick up 99.9%

of bacteria from the surface.